Even though the importance of vitamin fortification in milk and other dairy products is well documented and enforced, there is still a lot of leeway when it comes to the actual testing of the final levels of these supplements. The traditional method of testing for vitamins A and D in dairy by HPLC has a wide range of variability when it comes to testing. Final concentrations tested in milk samples have been noted to vary anywhere from 60-150% when tested between different labs and between technicians. The process of extracting vitamins for HPLC analysis is so long, cumbersome and difficult that there are numerous points in which error can occur. There’s been a need for a new methodology that can limit this range and provide reassurance that the levels tested are not only better than that 60-150% range, but drastically reduce this margin while maintaining precision. SciMed Technologies Inc. has developed the answer to this issue with their patented VitaKit’s®. With the use of monoclonal antibodies for specific binding and a quick/robust extraction procedure, variability is greatly reduced to 80-120% of the actual value. This is a smaller range than HPLC which gives more confidence in knowing that the final result is correct and reduces the need to repeat testing to be sure.